streptavidin pe Search Results


streptavidin pe  (SouthernBiotech)
94
SouthernBiotech streptavidin pe
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin Pe, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/Streptavidin-PE/pmc00145515-524-5-6
Average 94 stars, based on 1 article reviews
streptavidin pe - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
streptavidin pe conjugate  (Cytek Biosciences)
93
Cytek Biosciences streptavidin pe conjugate
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin Pe Conjugate, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/PE+Streptavidin/pm36172388-88-7-14
Average 93 stars, based on 1 article reviews
streptavidin pe conjugate - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
streptavidin r pe  (Qiagen)
94
Qiagen streptavidin r pe
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin R Pe, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/Streptavidin-R-PE/pm19654587-38-30-32
Average 94 stars, based on 1 article reviews
streptavidin r pe - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
streptavidin phycoerythrin pe cy7  (SouthernBiotech)
93
SouthernBiotech streptavidin phycoerythrin pe cy7
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin Phycoerythrin Pe Cy7, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/Streptavidin-PE%2FCY7/10__1128_slash_cvi__00338___12-115-18-20
Average 93 stars, based on 1 article reviews
streptavidin phycoerythrin pe cy7 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
phycoerythrin (pe)-conjugated streptavidin (10 g/ml)  (Becton Dickinson)
90
Becton Dickinson phycoerythrin (pe)-conjugated streptavidin (10 g/ml)
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Phycoerythrin (Pe) Conjugated Streptavidin (10 G/Ml), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/phycoerythrin++pe++conjugated+streptavidin++10+g+ml++antibody/10__1128_slash_iai__01280___12-60-7-40
Average 90 stars, based on 1 article reviews
phycoerythrin (pe)-conjugated streptavidin (10 g/ml) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pe-cy5 streptavidin  (Becton Dickinson)
90
Becton Dickinson pe-cy5 streptavidin
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Pe Cy5 Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/streptavidin+pe/pm32579975-528-35-40
Average 90 stars, based on 1 article reviews
pe-cy5 streptavidin - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pe-conjugated streptavidin  (Becton Dickinson)
90
Becton Dickinson pe-conjugated streptavidin
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Pe Conjugated Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/pe+conjugated+streptavidin/pm27534552-56-12-14
Average 90 stars, based on 1 article reviews
pe-conjugated streptavidin - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pe-labeled streptavidin  (Becton Dickinson)
90
Becton Dickinson pe-labeled streptavidin
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Pe Labeled Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/pe+labeled+streptavidin/pm11801655-56-10-11
Average 90 stars, based on 1 article reviews
pe-labeled streptavidin - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
streptavidin-pe and -allophycocyanin  (Becton Dickinson)
90
Becton Dickinson streptavidin-pe and -allophycocyanin
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin Pe And Allophycocyanin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/streptavidin+pe+and++allophycocyanin/pm16547252-53-11-16
Average 90 stars, based on 1 article reviews
streptavidin-pe and -allophycocyanin - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
streptavidin–r phycoerthryin (sa-pe)  (Columbia Biosciences)
90
Columbia Biosciences streptavidin–r phycoerthryin (sa-pe)
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin–R Phycoerthryin (Sa Pe), supplied by Columbia Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/streptavidin+phycoerythrin++sa+pe+/pmc05127142-114-0-6
Average 90 stars, based on 1 article reviews
streptavidin–r phycoerthryin (sa-pe) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
streptavidin–pe–cyanine 7 antibodies  (Becton Dickinson)
90
Becton Dickinson streptavidin–pe–cyanine 7 antibodies
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin–Pe–Cyanine 7 Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/streptavidin+pe+cyanine+7+antibodies/pmc02980998-67-37-43
Average 90 stars, based on 1 article reviews
streptavidin–pe–cyanine 7 antibodies - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
streptavidin pe-texas red  (Becton Dickinson)
90
Becton Dickinson streptavidin pe-texas red
Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and <t>streptavidin–APC,</t> followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.
Streptavidin Pe Texas Red, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+pe/streptavidin+pe+texasred/us10568913-315-64-71
Average 90 stars, based on 1 article reviews
streptavidin pe-texas red - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and streptavidin–APC, followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.

Journal:

Article Title: A novel function for the Tec family tyrosine kinase Itk in activation of ?1 integrins by the T-cell receptor

doi: 10.1093/emboj/20.6.1232

Figure Lengend Snippet: Fig. 4. The PH domain of Itk is required for Itk membrane redistribution upon CD3 stimulation of human T cells. (A) Jurkat T cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk and GFP–PH Itk were left unstimulated or CD3 stimulated for 10 min at 37°C. The cells were then visualized by confocal microscopy. Arrows highlight areas of punctate membrane localization of Itk. A minimum of 100 cells for each transfectant/stimulation condition were examined and three representative cells for each construct and stimulation condition are shown. (B) Jurkat T cells were left unstimulated or CD3 stimulated for 10 min at 37°C. Cells were stained with anti-Itk, biotin-conjugated rabbit anti-goat IgG and streptavidin–APC, followed by staining with FITC-conjugated cholera toxin B subunit. Representative cell images are shown, and a merged image demonstrating co-localization of endogenous Itk in DIGs following CD3 stimulation (in yellow) is shown. (C) Jurkat cells transiently transfected with GFP–wt Itk, GFP–ΔPH Itk or GFP–ΔPH Itk with a farnesylation sequence tag (F-GFP–ΔPH Itk) were left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. Cytosolic, membrane and DIG fractions were prepared as in Figure 1. Anti-HA immunoprecipitations were performed with lysates containing 5 × 106 GFP+ cell equivalents. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-HA antibody. (D) Jurkat cells transiently transfected with GFP–control, GFP–wt Itk, GFP–KN Itk or GFP–PH Itk were sorted and left unstimulated (U) or CD3 stimulated (CD3) for 5 min at 37°C. DIG fractions were prepared as in Figure 1. Anti-Itk immunoprecipitations were performed with lysates containing 3 × 106 cells. Immunoprecipitates were separated on a 7.5% SDS–polyacrylamide gel, transferred to PVDF and immunoblotted with an anti-phosphotyrosine mAb, stripped and reprobed with an anti-Itk antibody.

Article Snippet: Cells were then stained with streptavidin–PE (Southern Biotechnology Associates) at 1 µg/10 6 cells for 20 min at room temperature.

Techniques: Transfection, Confocal Microscopy, Construct, Staining, Sequencing

Fig. 9. CD3-induced actin polymerization is dependent on Itk. Jurkat T cells transiently transfected with vectors encoding either GFP, GFP–Itk (GFP–WT Itk), GFP–KN/Itk or GFP–PH Itk were sorted to isolate a homogeneous population of GFP+ T cells, and then left unstimulated (open curves) or CD3-stimulated for 10 min at 37°C (closed curves). Cells were stained with biotin-labeled phalloidin and PE-conjugated streptavidin, and analyzed by flow cytometry. Numbers in parentheses in each set of curves indicate the fold increase in mean fluorescence intensity of phalloidin staining upon CD3 stimulation.

Journal:

Article Title: A novel function for the Tec family tyrosine kinase Itk in activation of ?1 integrins by the T-cell receptor

doi: 10.1093/emboj/20.6.1232

Figure Lengend Snippet: Fig. 9. CD3-induced actin polymerization is dependent on Itk. Jurkat T cells transiently transfected with vectors encoding either GFP, GFP–Itk (GFP–WT Itk), GFP–KN/Itk or GFP–PH Itk were sorted to isolate a homogeneous population of GFP+ T cells, and then left unstimulated (open curves) or CD3-stimulated for 10 min at 37°C (closed curves). Cells were stained with biotin-labeled phalloidin and PE-conjugated streptavidin, and analyzed by flow cytometry. Numbers in parentheses in each set of curves indicate the fold increase in mean fluorescence intensity of phalloidin staining upon CD3 stimulation.

Article Snippet: Cells were then stained with streptavidin–PE (Southern Biotechnology Associates) at 1 µg/10 6 cells for 20 min at room temperature.

Techniques: Transfection, Staining, Labeling, Flow Cytometry, Fluorescence